Molecular Determination of H - antigens of Salmonella Using a 1 Microsphere Based Liquid Array
نویسندگان
چکیده
Microsphere Based Liquid Array 2 3 John R. McQuiston * , R. Jordan Waters, Blake A. Dinsmore, Matthew L. Mikoleit, and 4 Patricia I. Fields 5 Enteric Diseases Laboratory Branch, Division of Foodborne Waterborne and 6 Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. 7 8 9 10 Running Title: Molecular Determination of the H-antigens of Salmonella. 11 Corresponding Author: J.R. McQuiston, National Salmonella Reference Laboratory, 12 Division of Foodborne, Waterborne and Environmental Diseases 13 MS-C03, Centers for Disease Control and Prevention 14 1600 Clifton Rd., Atlanta, GA 30333 15 Phone: (404) 639-0270 16 Fax: (404) 639-3333 17 e-mail:[email protected] 18 19 Disclaimer 20 Use of trade names is for identification only and does not imply endorsement by the 21 Public Health Service or by the U.S. Department of Health and Human Services. 22 Copyright © 2010, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.01323-10 JCM Accepts, published online ahead of print on 15 December 2010
منابع مشابه
Isolation and Detection of Virulence factors of Salmonella typhimurium and Salmonella enteritidis
Plasmids play an important role in the virulence and antibiotic resistance of Salmonella. Salmonella carry plasmids having different size and number. Plasmid profile analysis of single isolates of Salmonella typhimurium and Salmonella enteritidis revealed the presence of 4- plasmid profiles. Salmonella serovars produce several Type III secretions including Sop-E and Sop –B. Soap E is detected b...
متن کاملDetermination of RT-PCR detection limit of live and dead Salmonella cells in raw and sterilized milk
The objective of the current study was to evaluate the reproducibility of a reverse transcriptase PCR (RT-PCR)-based technique to differentiate viable and dead Salmonella cells in raw and sterilized milk. The microorganism was initially inoculated into the milk samples followed by incubating at 37°C for 4 h prior to inactivation by heat at 80°C for 10 min. The treated and non-treated samples we...
متن کاملDetermination of somatic and excretory-secretory antigens of Fasciola hepatica and Fasciola gigantica using SDS-PAGE
Human fascioliasis due to unknown species and animal fascioliasis caused by both or one of Fasciola spp. are commonly seen in Iran. To compare electrophoretic patterns of somatic and excretory-secretory antigens of F. hepatica and F. gigantica by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the adult flukes were collected from infected slaughtered bovine livers. E/S a...
متن کاملSequence-level and dual-phase identification of Salmonella flagellum antigens by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Forty-three reference strains involving the 24 most common serovars of Salmonella enterica were examined by using a mass spectrometry-based H antigen typing platform (MS-H). The results indicate that MS-H can be used as a sensitive, rapid, and straightforward approach for the typing of Salmonella flagella at the molecular level without antiserum and phase inversion.
متن کاملImprovement the reflection coefficient of Waveguide-Fed Phased-Array Antenna utilizing Liquid Crystal
Abstract— This work investigates the improvement of the active reflection coefficient of waveguide-fed phased-array antenna using liquid crystal layers. The anisotropy properties of liquid crystal layer can be employed to eliminate blind scan angle and improve the wide angle impedance matching of the waveguide-fed phased array antennas. The authors have expressed the modal analysis of the waveg...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010